Direct detection of microorganisms in sonicated orthopedic devices after in vitro biofilm production and different processing conditions.
Juliette CieslinskiVictoria Stadler Tasca RibeiroLetícia KraftPaula Hansen SussEdvaldo RosaLuis Gustavo MorelloMarcelo PillonettoFelipe Francisco TuonPublished in: European journal of orthopaedic surgery & traumatology : orthopedie traumatologie (2021)
Microbial identification by MALDI-TOF in sonicated fluid is feasible. With qPCR, there were no differences between the detection in the screws processed immediately or after refrigeration. It is necessary to consider whether or not the refrigeration period would affect microbial recovery in an explanted prosthesis.