In vitro studies of the protein-interaction network of cell-wall lytic transglycosylase RlpA of Pseudomonas aeruginosa.
Luis F Avila-CobianStefania De BenedettiChoon KimRhona FeltzerMatthew M ChampionJed F FisherShahriar MobasheryPublished in: Communications biology (2022)
The protein networks of cell-wall-biosynthesis assemblies are largely unknown. A key class of enzymes in these assemblies is the lytic transglycosylases (LTs), of which eleven exist in P. aeruginosa. We have undertaken a pulldown strategy in conjunction with mass-spectrometry-based proteomics to identify the putative binding partners for the eleven LTs of P. aeruginosa. A total of 71 putative binding partners were identified for the eleven LTs. A systematic assessment of the binding partners of the rare lipoprotein A (RlpA), one of the pseudomonal LTs, was made. This 37-kDa lipoprotein is involved in bacterial daughter-cell separation by an unknown process. RlpA participates in both the multi-protein and multi-enzyme divisome and elongasome assemblies. We reveal an extensive protein-interaction network for RlpA involving at least 19 proteins. Their kinetic parameters for interaction with RlpA were assessed by microscale thermophoresis, surface-plasmon resonance, and isothermal-titration calorimetry. Notable RlpA binding partners include PBP1b, PBP4, and SltB1. Elucidation of the protein-interaction networks for each of the LTs, and specifically for RlpA, opens opportunities for the study of their roles in the complex protein assemblies intimately involved with the cell wall as a structural edifice critical for bacterial survival.
Keyphrases
- cell wall
- mass spectrometry
- binding protein
- protein protein
- pseudomonas aeruginosa
- stem cells
- small molecule
- gene expression
- liquid chromatography
- hiv testing
- human immunodeficiency virus
- mesenchymal stem cells
- bone marrow
- drug resistant
- candida albicans
- acinetobacter baumannii
- clinical evaluation
- tandem mass spectrometry