Generation 3 PAMAM dendrimer TAMRA conjugates containing precise dye/dendrimer ratios.
Janet ManonoCasey A DoughertyKirsten JonesJoshua DeMuthMark M Banaszak HollStassi DiMaggioPublished in: Materials today (Kidlington, England) (2015)
The synthesis, isolation, and characterization of generation 3 poly(amidoamine) (G3 PAMAM) dendrimer containing precise ratios of 5-carboxytetramethylrhodamine succinimidyl ester (TAMRA) dye (n = 1-3) per polymer particle are reported. Stochastic conjugation of TAMRA dye to the dendrimer was followed by separation into precise dye-polymer ratios using rp-HPLC. The isolated materials were characterized by rp-UPLC, MALDI-TOF-MS, and 1H NMR spectroscopy, UV-vis, and fluorescence spectroscopies.