Differential roles of RIG-I like receptors in SARS-CoV-2 infection.
Duo-Meng YangTing-Ting GengAndrew G HarrisonPeng-Hua WangPublished in: Military Medical Research (2021)
Retinoic acid-inducible gene I (RIG-I) and melanoma differentiation-associated protein 5 (MDA5) sense viral RNA and activate antiviral immune responses. Herein we investigate their functions in human epithelial cells, the primary and initial target of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). A deficiency in MDA5, RIG-I or mitochondrial antiviral signaling protein (MAVS) enhanced viral replication. The expression of the type I/III interferon (IFN) during infection was impaired in MDA5-/- and MAVS-/-, but not in RIG-I-/-, when compared to wild type (WT) cells. The mRNA level of full-length angiotensin-converting enzyme 2 (ACE2), the cellular entry receptor for SARS-CoV-2, was ~ 2.5-fold higher in RIG-I-/- than WT cells. These data demonstrate MDA5 as the predominant SARS-CoV-2 sensor, IFN-independent induction of ACE2 and anti-SARS-CoV-2 role of RIG-I in epithelial cells.
Keyphrases
- sars cov
- respiratory syndrome coronavirus
- cell cycle arrest
- angiotensin converting enzyme
- immune response
- angiotensin ii
- breast cancer cells
- cell death
- induced apoptosis
- dendritic cells
- pi k akt
- wild type
- binding protein
- coronavirus disease
- endothelial cells
- genome wide
- copy number
- deep learning
- big data
- genome wide identification
- cell proliferation