Single-molecule detection assisted by the target-triggered signal amplification strategy for ultrasensitive quantitative analysis of intracellular telomerase activity.
Chengfang JiangQi WangJing GengMengmeng LiYuqi ZhangXinli ShiYan ZhangXinyue SongShu-Sheng ZhangPublished in: Chemical communications (Cambridge, England) (2024)
We developed a multiplex single-molecule quantitative assay of intracellular telomerase that used target-triggered signal amplification to enhance sensitivity, substrate reaction to increase signal stability, and quantum dots to enhance signal-to-noise ratio, obtaining an LOD of 5 × 10 -14 IU for intracellular telomerase and LOD of 3 cells for multiple cancer cells.
Keyphrases
- single molecule
- quantum dots
- atomic force microscopy
- living cells
- label free
- reactive oxygen species
- induced apoptosis
- high throughput
- real time pcr
- gold nanoparticles
- nucleic acid
- high resolution
- sensitive detection
- signaling pathway
- endoplasmic reticulum stress
- oxidative stress
- molecularly imprinted
- simultaneous determination
- amino acid
- structural basis