A Practical Method for Amino Acid Analysis by LC-MS Using Precolumn Derivatization with Urea.
Runjin ZhaoBiling HuangGang LuSongsen FuJianxi YingYufen ZhaoPublished in: International journal of molecular sciences (2023)
Amino acid (AA) analysis is important in biochemistry, food science, and clinical medicine. However, due to intrinsic limitations, AAs usually require derivatization to improve their separation and determination. Here, we present a liquid chromatography-mass spectrometry (LC-MS) method for the derivatization of AAs using the simple agent urea. The reactions proceed quantitatively under a wide range of conditions without any pretreatment steps. Urea-derivatized products (carbamoyl amino acids) of 20 AAs exhibit better separation on reversed-phase columns and increased response in a UV detector compared to underivatized ones. We applied this approach to AA analysis in complex samples using a cell culture media as a model, and it showed potential for the determination of oligopeptides. This fast, simple, and inexpensive method should be useful for AA analysis in complex samples.
Keyphrases
- liquid chromatography
- mass spectrometry
- amino acid
- high resolution mass spectrometry
- tandem mass spectrometry
- ms ms
- high performance liquid chromatography
- simultaneous determination
- solid phase extraction
- liquid chromatography tandem mass spectrometry
- risk assessment
- gas chromatography mass spectrometry
- climate change
- monte carlo