Knockdown of LMNA inhibits Akt/β-catenin-mediated cell invasion and migration in clear cell renal cell carcinoma cells.
Hui XinYu TangYan-Hong JinHu-Li LiYu TianCong YuZe-Ju ZhaoMing-Song WuYou-Fu PanPublished in: Cell adhesion & migration (2023)
The LMNA gene encoding lamin A/C is amplified in some clear cell renal cell carcinoma (ccRCC) samples. Our data showed that depletion of the tumor suppressor PBRM1 can upregulate lamin A/C levels, and lamin A/C could interact with PBRM1. However, the role of lamin A/C in ccRCC is not yet fully understood. Our functional assays showed that although the proliferation ability was slightly impaired after LMNA depletion, the migration and invasion of ccRCC cells were significantly inhibited. This suppression was accompanied by a reduction in MMP2, MMP9, AKT/p-AKT, and Wnt/β-catenin protein levels. Our data therefore suggest that lamin A/C, as an interaction partner of the tumor suppressor PBRM1, plays a crucial role in tumor invasion and metastasis in ccRCC.
Keyphrases
- cell proliferation
- signaling pathway
- induced apoptosis
- cell migration
- epithelial mesenchymal transition
- muscular dystrophy
- electronic health record
- clear cell
- big data
- pi k akt
- stem cells
- cell cycle arrest
- single cell
- binding protein
- oxidative stress
- genome wide
- artificial intelligence
- bone marrow
- hiv infected
- deep learning
- genome wide identification