Functional dissection of neural circuitry using a genetic reporter for fMRI.
Souparno GhoshNan LiMiriam SchwalmBenjamin B BartelleTianshu XieJade I DaherUrvashi D SinghKatherine XieNicholas DiNapoliNicholas B EvansKwanghun ChungAlan JasanoffPublished in: Nature neuroscience (2022)
The complex connectivity of the mammalian brain underlies its function, but understanding how interconnected brain regions interact in neural processing remains a formidable challenge. Here we address this problem by introducing a genetic probe that permits selective functional imaging of distributed neural populations defined by viral labeling techniques. The probe is an engineered enzyme that transduces cytosolic calcium dynamics of probe-expressing cells into localized hemodynamic responses that can be specifically visualized by functional magnetic resonance imaging. Using a viral vector that undergoes retrograde transport, we apply the probe to characterize a brain-wide network of presynaptic inputs to the striatum activated in a deep brain stimulation paradigm in rats. The results reveal engagement of surprisingly diverse projection sources and inform an integrated model of striatal function relevant to reward behavior and therapeutic neurostimulation approaches. Our work thus establishes a strategy for mechanistic analysis of multiregional neural systems in the mammalian brain.
Keyphrases
- resting state
- functional connectivity
- white matter
- deep brain stimulation
- magnetic resonance imaging
- living cells
- parkinson disease
- quantum dots
- genome wide
- sars cov
- induced apoptosis
- multiple sclerosis
- obsessive compulsive disorder
- high resolution
- copy number
- computed tomography
- gene expression
- magnetic resonance
- drinking water
- cell death
- dna methylation
- signaling pathway
- cell cycle arrest
- contrast enhanced