Fluorinated trimethyllysine as a 19F NMR probe for trimethyllysine hydroxylase catalysis.

Y Vijayendar ReddyAbbas H K Al TemimiJasmin Mecinovic

Published in: Organic & biomolecular chemistry (2018)

Trimethyllysine hydroxylase (TMLH) catalyses C-3 hydroxylation of Nε-trimethyllysine in the first step of carnitine biosynthesis in humans. Studies on TMLH have been hampered by the lack of established chemical methods. We report that an Nε-trimethyllysine analogue that contains the fluoromethyl group can be used as a 1H and 19F NMR probe for studies on TMLH catalysis.

Keyphrases

magnetic resonance
high resolution
living cells
case control
quantum dots
solid state
mass spectrometry
single molecule