Quercetin inhibits gout arthritis in mice: induction of an opioid-dependent regulation of inflammasome.
Kenji W Ruiz-MiyazawaLarissa Staurengo-FerrariSandra S MizokamiTalita P DomicianoFabiana T M C VicentiniDoumit Camilios-NetoWander R PavanelliPhileno Pinge-FilhoFlávio A AmaralMauro M TeixeiraRubia CasagrandeWaldiceu Aparecido VerriPublished in: Inflammopharmacology (2017)
We investigated the anti-inflammatory and analgesic effects of quercetin in monosodium urate crystals (MSU)-induced gout arthritis, and the sensitivity of quercetin effects to naloxone, an opioid receptor antagonist. Mice were treated with quercetin, and mechanical hyperalgesia was assessed at 1-24 h after MSU injection. In vivo, leukocyte recruitment, cytokine levels, oxidative stress, NFκB activation, and gp91phox and inflammasome components (NLRP3, ASC, Pro-caspase-1, and Pro-IL-1β) mRNA expression by qPCR were determined in the knee joints at 24 h after MSU injection. Inflammasome activation was determined, in vitro, in lipopolysaccharide-primed macrophages challenged with MSU. Quercetin inhibited MSU-induced mechanical hyperalgesia, leukocyte recruitment, TNFα and IL-1β production, superoxide anion production, inflammasome activation, decrease of antioxidants levels, NFκB activation, and inflammasome components mRNA expression. Naloxone pre-treatment prevented all the inhibitory effects of quercetin over MSU-induced gout arthritis. These results demonstrate that quercetin exerts analgesic and anti-inflammatory effect in the MSU-induced arthritis in a naloxone-sensitive manner.
Keyphrases
- anti inflammatory
- diabetic rats
- oxidative stress
- rheumatoid arthritis
- high glucose
- neuropathic pain
- signaling pathway
- lps induced
- uric acid
- cell death
- type diabetes
- pain management
- spinal cord injury
- endothelial cells
- high resolution
- ionic liquid
- knee osteoarthritis
- insulin resistance
- hydrogen peroxide
- ultrasound guided
- mass spectrometry
- nitric oxide
- heat shock protein
- anterior cruciate ligament