Tracking endoplasmic reticulum viscosity during ferroptosis and autophagy using a molecular rotor probe.
Akshay SilswalApurba Lal KonerPublished in: Chemical communications (Cambridge, England) (2023)
Ferroptosis is a unique non-apoptotic cell death process associated with endoplasmic reticulum (ER) stress-related diseases. We have designed and synthesized a far-red emitting and ER targetable viscosity-sensitive fluorophore to track ER-phagy. Furthermore, the ER viscosity alteration during the ferroptosis process was investigated via intensity and lifetime-based spectroscopy and microscopy.
Keyphrases
- endoplasmic reticulum
- cell death
- single molecule
- cell cycle arrest
- high resolution
- quantum dots
- fluorescent probe
- living cells
- atomic force microscopy
- high intensity
- high speed
- high throughput
- optical coherence tomography
- mass spectrometry
- signaling pathway
- oxidative stress
- endoplasmic reticulum stress
- cell proliferation
- label free
- drug induced
- energy transfer