Ratiometric Electrochemical Switch for Circulating Tumor DNA through Recycling Activation of Blocked DNAzymes.
Hua ChaiYuguo TangZhenzhen GuoPeng MiaoPublished in: Analytical chemistry (2022)
Circulating tumor DNA (ctDNA) serves as a powerful noninvasive and viable biomarker for the diagnosis of cancers. The abundance of ctDNA in patients with advanced stages is significantly higher than that in patients with early stages. Herein, a ratiometric electrochemical biosensor for the detection of ctDNA is developed by smart design of DNA probes and recycles of DNAzyme activation. The conformational variation of DNA structures leads to the changes of two types of electrochemical species. This enzyme-free sensing strategy promotes excellent amplification efficiency upon target recognition. The obtained results assure good analytical performances and a limit of detection as low as 25 aM is achieved. Additionally, this method exhibits outstanding selectivity and great application prospects in biological sample analysis.
Keyphrases
- circulating tumor
- label free
- cell free
- circulating tumor cells
- gold nanoparticles
- living cells
- sensitive detection
- quantum dots
- fluorescent probe
- ionic liquid
- single molecule
- molecularly imprinted
- small molecule
- molecular dynamics
- hydrogen peroxide
- real time pcr
- high resolution
- nucleic acid
- molecular dynamics simulations
- photodynamic therapy
- liquid chromatography
- microbial community