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In vivo biosynthesis of tyrosine analogs and their concurrent incorporation into a residue-specific manner for enzyme engineering.

Yumi WonHyunwoo JeonAmol D PagarMahesh D PatilSaravanan Prabhu NadarajanDillon T FloodPhillip E DawsonHyungdon Yun
Published in: Chemical communications (Cambridge, England) (2019)
Herein we report the development of an efficient cellular system for the in vivo biosynthesis of Tyr-analogs and their concurrent incorporation into target proteins by the residue-specific approach. This system makes use of common phenol derivatives and the tyrosine phenol lyase machinery to create various tyrosine analogues that impart desired properties on the target proteins. Biosynthesized 2-fluorotyrosine was incorporated into three industrially important enzymes which resulted in enhanced thermostability.
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