siRNA-Mediated MELK Knockdown Induces Accelerated Wound Healing with Increased Collagen Deposition.
Lukasz SzymanskiSławomir LewickiTomasz MarkiewiczSzczepan CierniakJean-Pierre TassanJacek Z KubiakPublished in: International journal of molecular sciences (2023)
Skin wounds remain a significant problem for the healthcare system, affecting the clinical outcome, patients' quality of life, and financial costs. Reduced wound healing times would improve clinical, economic, and social aspects for both patients and the healthcare system. Skin wound healing has been studied for years, but effective therapy that leads to accelerated wound healing remains to be discovered. This study aimed to evaluate the potential of MELK silencing to accelerate wound healing. A vectorless, transient knockdown of the MELK gene using siRNA was performed in a murine skin wound model. The wound size, total collagen, type 3 collagen, vessel size, vessel number, cell proliferation, cell apoptosis, number of mast cells, and immune infiltration by CD45, CD11b, CD45, and CD8a cells were evaluated. We observed that treatment with MELK siRNA leads to significantly faster wound closing associated with increased collagen deposition.
Keyphrases
- wound healing
- cell proliferation
- end stage renal disease
- ejection fraction
- chronic kidney disease
- newly diagnosed
- prognostic factors
- healthcare
- stem cells
- peritoneal dialysis
- mental health
- gene expression
- patient reported outcomes
- patient reported
- cell cycle
- drug delivery
- risk assessment
- oxidative stress
- copy number
- climate change
- smoking cessation
- replacement therapy
- cerebral ischemia