Real-time detection and imaging of copper(ii) in cellular mitochondria.
Hong LiRuilong ZhangChunxia LiBei HuangTingting YuXiaodan HuangXuanjun ZhangFei LiHongping ZhouYupeng TianPublished in: Organic & biomolecular chemistry (2018)
A triphenylamine-pyridine derivative, viz., a fluorescence probe was designed and synthesized. The probe displayed an "on-off-on" fluorescence switch, upon the sequential addition of Cu2+ and ethylenediaminetetraacetic acid (EDTA). The detection mechanism was confirmed by using UV-vis, fluorescence and 1H NMR titration spectra. Interestingly, the probe can accumulate in mitochondria, and thus detect Cu2+ in real-time there, which indicated that probe L can be used to monitor intracellular mitochondrial Cu2+ levels. Further understanding of the biological functions of Cu2+ in mitochondria has important implications for treating cancer and other diseases.
Keyphrases
- living cells
- single molecule
- aqueous solution
- quantum dots
- reactive oxygen species
- energy transfer
- cell death
- high resolution
- metal organic framework
- endoplasmic reticulum
- loop mediated isothermal amplification
- fluorescent probe
- oxidative stress
- label free
- magnetic resonance
- real time pcr
- papillary thyroid
- squamous cell
- sensitive detection
- squamous cell carcinoma
- density functional theory
- childhood cancer
- solid state