Role of chromium reductases, antioxidants, and biosorption against oxidative damage of metals by Bacillus cereus.

Current research was performed to look for the performance of Bacillus cereus PY3 for metal detoxification. Strain PY3 was recognized as B. cereus using 16 S rRNA. Higher rate of removal of Zn and Cr (VI) by PY3 was obtained between pH 6-8 and 100-500 µg/mL in 24 h. Highest removal of Cr 6+ by strain PY3 was achieved at acidic, neutral, and alkaline atmosphere, 100-300 µg Cr 6+ /mL and 25-35°C. Supernatant of PY3 detoxified Cr 6+ into Cr 3+ then cell pellet (debris) adsorbed them. The mechanism of metal removal was due to the release of cytolic extracts. Release of antioxidants and bio-film played a protective role against cell damage. Metals increased antioxidants and bio-film formation. SEM images showed the smooth external structure of PY3 when cells were exposed to metals thus confirming the role of cells for detoxification. Results Above facts conclude that PY3 can remove metallic pollution in polluted soil.