A pH-activated fluorescent probe via transformation of azo and hydrazone forms for lysosomal pH imaging.
Xiao-Lei ZhaoChenxu YanWan-Shan QiuTao YuWu-Jin WangWei-Hong ZhuPublished in: Chemical communications (Cambridge, England) (2022)
We developed a fluorescent probe Sth-NH by introducing a 6-hydroxypyridone skeleton. The presence of an active proton enables the probe to transform from a deprotonated azo form to a hydrazone form in a strongly acidic environment to realize fluorescence light-up behavior, thus monitoring the lower lysosomal pH of cancer cells and distinguishing them from normal cells.