Wharton's jelly mesenchymal stem cell-based or umbilical vein endothelial cell-based serum-free coculture with cytokines supports the ex vivo expansion/maintenance of cord blood hematopoietic stem/progenitor cells.

Qiuyang LiDewan ZhaoQiang ChenMaowen LuoJingcao HuangCao YangFangfang WangWenxian LiTing Liu

Published in: Stem cell research & therapy (2019)

Our data demonstrate that both WJ-MSCs and UVECs as feeder layer could efficiently support the expansion of UCB-CD34+ cells in synergy with SCF, FLT3L, and TPO under serum-free culture condition. The UVECs combined with the 3GF cytokine cocktail could maintain the growth of LTC-ICs derived from UCB-CD34+ cells and even expand to some extent.

Keyphrases

induced apoptosis
cord blood
mesenchymal stem cells
cell cycle arrest
umbilical cord
endothelial cells
acute myeloid leukemia
cell death
oxidative stress
endoplasmic reticulum stress
stem cells
signaling pathway
bone marrow
machine learning
electronic health record
tyrosine kinase
cell therapy
nk cells
data analysis