A Combination of Three Antioxidants Decreases the Impact of Rural Particulate Pollution in Normal Human Keratinocytes.

Specific signaling pathways known to be correlated with skin inflammation and aging were examined based on their suitability for use in efficacy testing for the prevention of skin damage due to ambient hydrocarbon pollution. Endpoints examined after only 5 hours of exposure provide a useful method amenable to high through-put screening. The results obtained reinforce the concept that a multiple antioxidant preparation, topically applied, may reduce pro-inflammatory signaling and cellular damage and thereby reduce premature skin aging due to exposure to rural-derived airborne pollution.