Human DNA decays faster with time than viral dsDNA: an analysis on HPV16 using pathology archive samples spanning 85 years.

Sara Nicolás-PárragaMontserrat TorresLaia AlemanyAna FélixEugenia CruzSilvia de SanjoséFrancesc Xavier BoschIgnacio G Bravonull null

Published in: Virology journal (2021)

We conclude that human and viral DNA show a differential decay kinetics in FFPE samples. The faster degradation of human DNA should be considered when assessing viral DNA prevalence in long stored samples, as HPV DNA detection remains a key biomarker of viral-associated transformation.

Keyphrases

circulating tumor
endothelial cells
cell free
single molecule
sars cov
induced pluripotent stem cells
nucleic acid
risk factors
circulating tumor cells
loop mediated isothermal amplification