Hand-Held and Integrated Tubular Tip-like Sensing Platform Series: Point-of-care Device for Semi-automated Multiplexed Assay.

Currently, most of the electrochemical sensors were prepared based on the planar electrode (PE) and utilized in open circumstance. The accompanying issues include fixed and limited sensing area of PE, insufficient usage of the testing sample, tedious operation, and susceptibility to external environment. Herein, a novel tubular tip-like sensor (TTLS) platform was proposed, where a small tip accommodates all electrodes with a curved surface and also acts as a closed detection chamber. Teaming up with a commercial pipette and potentiostat, the TTLS is able to accomplish the whole assay procedure including sampling, detection, rinsing, and regeneration with a single hand. The electrochemical interface area can be easily tuned to adapting for different scenarios with varied sensitivity request. Moreover, two TTLS-based array systems were derived: one integrates multiple working electrodes in one tip for multicomponent quantification and the other assembles eight independent TTLSs for high-throughput analysis. The admirable sensing performance of the TTLS was fully proved by detecting several liver-related biomarkers in 5 μL of the serum sample. The proposed tubular sensor platform is superior to the traditional electrochemical sensor in the aspects of unique sensing surface, fast and simple operation, good portability, and great compatibility. The TTLS could be used as an ideal analytical tool in point-of-care testing and other fields.