Natural Molybdenite- and Tyrosinase-Based Amperometric Catechol Biosensor Using Acridine Orange as a Glue, Anchor, and Stabilizer for the Adsorbed Tyrosinase.
Yan ZhangYue WangZhiqiang ZhangAhmed SobhySusumu SatoMasaya UchidaYasushi HasebePublished in: ACS omega (2021)
To develop a natural mineral-based electrochemical enzyme biosensor, natural molybdenite (MLN), tyrosinase (TYR), and acridine orange (AO) were coadsorbed onto a glassy carbon electrode (GCE). The developed TYR/AO/MLN-GCE-based amperometric TYR biosensor exhibited excellent performance for highly sensitive determination of catechol (linear range, 0.1-80 μM; sensitivity, 0.0315 μA/μM; LOD, 0.029 μM; response time, <4 s) with good reproducibility and good operational and storage stabilities. The electrochemical impedance spectroscopy (EIS) and quartz crystal microbalance with dissipation (QCM-D) revealed interesting roles of AO: (1) an efficient glue for enhancing the amount of the adsorbed TYR on the MLN-GCE, (2) an anchor for efficient orientation of the adsorbed TYR on the MLN-GCE, and (3) a stabilizer providing a suitable microenvironment for the adsorbed TYR on the MLN-GCE surface. This physical adsorption-based AO-coupled enzyme-modification strategy onto natural MLN would be a versatile strategy to develop cost-effective and environment-friendly natural mineral-based electrochemical biosensors and bioelectronic devices.
Keyphrases
- label free
- gold nanoparticles
- molecularly imprinted
- sensitive detection
- reduced graphene oxide
- quantum dots
- ionic liquid
- hydrogen peroxide
- stem cells
- mental health
- mass spectrometry
- high resolution
- computed tomography
- single molecule
- magnetic resonance
- liquid chromatography
- electron transfer
- tandem mass spectrometry