Proteome analysis of the salivary pellicle formed on titanium alloys containing niobium and zirconium.
Heloisa Navarro PantarotoKarina Pintaudi AmorimJairo Matozinho CordeiroJoão Gabriel S SouzaAntonio Pedro Ricomini FilhoElidiane C RangelAna Lúcia R RibeiroLuís Geraldo VazValentim Adelino Ricardo BarãoPublished in: Biofouling (2019)
The chemical composition of biomaterials can drive their biological responses; therefore, this in vitro study aimed to evaluate the proteomic profile of the salivary pellicle formed on titanium (Ti) alloys containing niobium (Nb) and zirconium (Zr). The experimental groups consisted of Ti35NbxZr (x = 5 and 10 wt%) alloys, and commercially pure titanium (cpTi); titanium aluminium vanadium (Ti6Al4V) alloys were used as controls. The physical and chemical characteristics of the Ti materials were analysed. The proteomic profile was evaluated by liquid chromatography coupled with tandem mass spectrometry. Bacterial adhesion (2 h) of mixed species (Streptococcus sanguinis and Actinomyces naeslundii) was investigated as colony-forming units (n = 6). This paper reports the finding that salivary pellicle composition can be modulated by the composition of the Ti material. The Ti35NbxZr group showed a significant ability to adsorb proteins from saliva, which can favour interactions with cells and compatibility with the body.
Keyphrases
- tandem mass spectrometry
- liquid chromatography
- ultra high performance liquid chromatography
- mass spectrometry
- high performance liquid chromatography
- simultaneous determination
- high resolution mass spectrometry
- gas chromatography
- induced apoptosis
- high resolution
- mental health
- emergency department
- computed tomography
- biofilm formation
- solid phase extraction
- signaling pathway
- label free
- bone regeneration