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A lysosome-targetable fluorescent probe for the simultaneous sensing of Cys/Hcy and GSH from different emission channels.

Hui ZhangLizhen XuWenxiu LiWen-Qiang ChenShan HuangJun HuangChusheng HuangJiarong ShengXiangzhi Song
Published in: RSC advances (2019)
A lysosome-specific fluorescent probe, Lyso-AC, for biothiols was developed by incorporation of a 4-nitrophenol moiety into a coumarin dye. The Cys/Hcy-triggered substitution-rearrangement cascade, and GSH-induced substitution reaction lead to the corresponding blue emissive amino-coumarin and yellow emissive thiol-coumarin, thereby enabling Cys/Hcy and GSH detection from distinct emissions. Moreover, this probe displayed an excellent lysosome targeting property with a 0.92 Pearson's colocalization coefficient by using Neutral Red as a reference. Significantly, biological experiments indicated Lyso-AC has the potential to monitor lysosome Cys/Hcy and GSH simultaneously in living HeLa cells from distinct emissions.
Keyphrases
  • fluorescent probe
  • living cells
  • light emitting
  • high glucose
  • cancer therapy
  • computed tomography
  • magnetic resonance imaging
  • climate change
  • highly efficient
  • oxidative stress
  • single molecule
  • contrast enhanced