A Direct Method for RT-PCR Detection of SARS-CoV-2 in Clinical Samples.

Sherif Aly El-KafrawyMai M El-DalyAhmed M HassanReham M KakiAdel M AbuzenadahMohammad Amjad Kamal Esam Ibraheem Azhar

Published in: Healthcare (Basel, Switzerland) (2021)

the direct PCR technique was found to be a reliable and sensitive method that can be used to reduce the time and cost of the assay by removing the need for RNA extraction. It enables the use of the assay in research, diagnostics, and screening for COVID-19 in regions with fewer economic resources, where supplies are more limited allowing for wider use for screening.

Keyphrases

sars cov
real time pcr
high throughput
respiratory syndrome coronavirus
coronavirus disease
loop mediated isothermal amplification
sensitive detection
life cycle