Pyridine-pyrazole based Al(iii) 'turn on' sensor for MCF7 cancer cell imaging and detection of picric acid.
Sayan SahaAvik DeArijit GhoshAvik GhoshKaushik BeraKrishna Sundar DasSohel AkhtarNakul C MaitiAbhijit Kumar DasBenu Brata DasRaju MondalPublished in: RSC advances (2021)
We report herein the development of a new pyridine-pyrazole based bis-bidentate asymmetric chemosensor that shows excellent turn-on chelation-enhanced Al 3+ -responsive fluorescence. The presence of two 'hard' phenolic hydroxyl groups plays a pivotal role in switching-on the sensing through coordination to the 'hard' Al 3+ ion, while the mechanism can be interpreted by the chelation-enhanced fluorescence (CHEF) process. The X-ray single structure show a planar conjugated structure of the ligand, which was further stabilized by extensive H-bonding and π-π stacking. The photophysical studies related to the sensing behavior of the titular ligand toward aluminum was investigated in detail using various spectroscopic techniques like UV-Vis, photoluminescence, fluorescence and time-correlated single-photon count (TCSPC) and time-resolved NMR. The spectroscopic methods also confirm the selective detection of Al 3+ ion in the presence of other metal ions. The theoretical calculations using Density Functional Theory (DFT) and the Time Dependent Density Functional Theory (TD-DFT) provide further insight on the mechanistic aspects of the turn-on sensing behavior including the electronic spectra of both the ligand and the complex. Interestingly, the as-synthesized H 2 DPC-Al complex can also be utilized as a fluorescence-based sensor for various nitroaromatics including picric acid, for which an INHIBIT logic gate can also be constructed. The as synthesized complex was subsequently used as a fluorescent probe for imaging of human breast adenocarcinoma (MCF7) cells using live cell confocal microscopic techniques.
Keyphrases
- density functional theory
- fluorescent probe
- living cells
- molecular dynamics
- molecular docking
- single molecule
- high resolution
- energy transfer
- quantum dots
- sensitive detection
- magnetic resonance
- breast cancer cells
- induced apoptosis
- endothelial cells
- squamous cell carcinoma
- computed tomography
- label free
- wastewater treatment
- radiation therapy
- molecular dynamics simulations
- cell cycle arrest
- signaling pathway
- cell death
- solid state
- cell proliferation
- atomic force microscopy
- high speed
- cancer therapy
- ionic liquid
- real time pcr
- raman spectroscopy
- induced pluripotent stem cells