Optical fiber sensors are one preferred solution for temperature sensing, especially for their capability of real-time monitoring and remote detection. However, many of them still suffer from a huge sensing system and complicated signal demodulate process. In order to solve these problems, we propose a smartphone-based optical fiber fluorescence temperature sensor. All the components, including the laser, filter, fiber coupler, batteries, and smartphone, are integrated into a 3D-printed shell, on the side of which there is a fiber flange used for the sensing probe connection. The fluorescence signal of the rhodamine B solution encapsulated in the sensing probe can be captured by the smartphone camera and extracted into the R value and G value by a self-developed smartphone application. The temperature can be quantitatively measured by the calibrated G/R-temperature relation, which can be unified using the same linear relationship in all solid-liquid-gas environments. The performance verifications prove that the sensor can measure temperature in high accuracy, good stability and repeatability, and has a long conservation time for at least 3 months. The proposed sensor not only can measure the temperature for remote and real-time detection needs, but it is also handheld with a small size of 167 mm × 85 mm × 75 mm supporting on-site applications. It is a potential tool in the temperature sensing field.