Mixed IgG Fc immune complexes exhibit blended binding profiles and refine FcR affinity estimates.
Zhixin Cyrillus TanAnja LuxMarkus BiburgerPrabha VargheseStephen LeesFalk NimmerjahnAaron S MeyerPublished in: bioRxiv : the preprint server for biology (2023)
The binding behavior of mixed Fc immune complexes is a blend of the binding properties for each constituent IgG species.An equilibrium, multivalent binding model can be generalized to incorporate immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions, immune complexes provide better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice.