Virulence Gene Profiles and Clonal Relationships of Escherichia coli O26:H11 Isolates from Feedlot Cattle as Determined by Whole-Genome Sequencing.
Narjol Gonzalez-EscalonaMagaly ToroLydia V RumpGuojie CaoT G NagarajaJianghong MengPublished in: Applied and environmental microbiology (2016)
Escherichia coli O26 is the second most important type of enterohemorrhagic E. coli (EHEC) worldwide. Serogroup O26 strains are categorized into two groups: enteropathogenic (EPEC) carrying LEE, causing mild diarrhea, and Shiga toxigenic (STEC) carrying the stx gene, responsible for more severe outcomes. However, there are currently problems in distinguishing one group from the other. Furthermore, several O26 stx-negative strains are consistently misidentified as either EHEC-like or EPEC. The use of whole-genome sequence (WGS) analysis of O26 strains from cattle in the United States (i) allowed identification of O26 strains present in U.S. cattle, (ii) determined O26 strain diversity, (iii) solved the misidentification problem, and (iv) screened for the presence of antimicrobial resistance and virulence genes in the strains. This study provided a framework showing how to easily and rapidly use WGS information to identify and genetically characterize E. coli O26:H11, which is important for food safety and public health.
Keyphrases
- escherichia coli
- antimicrobial resistance
- public health
- biofilm formation
- klebsiella pneumoniae
- genome wide
- copy number
- genome wide identification
- mental health
- type diabetes
- healthcare
- gene expression
- staphylococcus aureus
- early onset
- metabolic syndrome
- insulin resistance
- clostridium difficile
- transcription factor
- social media
- weight loss
- human health
- climate change
- health information
- type iii