Genetic diversity, LC-ESI-MS chemical profile and in vivo antitumor activity of three Egyptian soybean cultivars.
Essam A Abdel-SattarAzza Ramy Abdel-MonemMohamed-Elamir F HegazyAli M El-HalawanySherif Mahmoud AfifiPublished in: Natural product research (2019)
Soybean (Glycine max L.) is one of the most important and widely consumed food plants worldwide. The objective of this study was to investigate the metabolite profiling of three Egyptian soybean cultivars (Giza 22, Giza 35 and Giza 111) and their in vivo antitumor effect. Random amplified polymorphic DNA (RAPD) analysis developed polymorphism level of 75% in 72 distinct markers. Applying LC-ESI-MS analysis, twenty-nine metabolites were recognized from the 80% methanol extract of all cultivars. In vivo antitumor activity of the 80% methanolic extract against solid Ehrlich ascites carcinoma (EAC) inoculated in mice model, showed a significant diminishing in tumor volume and reduced Glutathione (rGSH) and a significant increase in malondialdehyde (MDA) which was supported by histopathological examination. Among the studied cultivars, Giza 22 cultivar contained the highest total phenolic content (TPC) that may contribute to its impressive antioxidant capacity and antitumor activity.
Keyphrases
- ms ms
- mass spectrometry
- genetic diversity
- multiple sclerosis
- oxidative stress
- simultaneous determination
- cell free
- single cell
- type diabetes
- liquid chromatography
- adipose tissue
- anti inflammatory
- metabolic syndrome
- cell proliferation
- single molecule
- breast cancer cells
- insulin resistance
- solid phase extraction
- circulating tumor cells
- neural network
- human health