14-3-3ε protein-immobilized PCL-HA electrospun scaffolds with enhanced osteogenicity.
G RiveroA A AldanaY R Frontini LopezL LiveraniA R BoccaciniD M BustosGustavo Abel AbrahamPublished in: Journal of materials science. Materials in medicine (2019)
Adipose-derived mesenchymal stem cells (ASCs) accelerate the osteointegration of bone grafts and improve the efficiency in the formation of uniform bone tissue, providing a practical and clinically attractive approach in bone tissue regeneration. In this work, the effect of nanofibrous biomimetic matrices composed of poly(ε-caprolactone) (PCL), nanometric hydroxyapatite (nHA) particles and 14-3-3 protein isoform epsilon on the initial stages of human ASCs (hASCs) osteogenic differentiation was investigated. The cells were characterized by flow cytometry and induction to differentiation to adipogenic and osteogenic lineages. The isolated hASCs were induced to differentiate to osteoblasts over all scaffolds, and adhesion and viability of the hASCs were found to be similar. However, the activity of alkaline phosphatase (ALP) as early osteogenic marker in the PCL-nHA/protein scaffold was four times higher than in PCL-nHA and more than five times than the measured in neat PCL.
Keyphrases
- tissue engineering
- mesenchymal stem cells
- bone marrow
- flow cytometry
- bone mineral density
- protein protein
- bone regeneration
- soft tissue
- endothelial cells
- stem cells
- induced apoptosis
- bone loss
- high glucose
- cell death
- cystic fibrosis
- adipose tissue
- escherichia coli
- ionic liquid
- staphylococcus aureus
- cell cycle arrest
- biofilm formation
- diabetic rats
- capillary electrophoresis
- high resolution