A Full-Length Infectious cDNA Clone of Zika Virus from the 2015 Epidemic in Brazil as a Genetic Platform for Studies of Virus-Host Interactions and Vaccine Development.

Konstantin A TsetsarkinHeather KenneyRubing ChenGuangping LiuHasmik ManukyanStephen S WhiteheadMajid LaassriKonstantin ChumakovAlexander G Pletnev

Published in: mBio (2016)

The availability of genetic tools and laboratory models determines the progress in understanding mechanisms of virus emergence and pathogenesis. Recent large-scale outbreaks of Zika virus (ZIKV) that were linked to complications during perinatal development and Guillain-Barré syndrome in adults emphasize the urgency for the development of a reverse-genetics system based on an epidemic ZIKV strain. Here, we report a stable infectious cDNA clone for ZIKV isolated during the 2015 epidemic in Brazil, as well as a Vero cell-adapted version of it, which will be used for virus-host interaction studies and vaccine development.

Keyphrases

zika virus
dengue virus
aedes aegypti
single cell
stem cells
gene expression
risk factors
high throughput
cell therapy
urinary incontinence