US FDA-validated green GC-MS method for analysis of gabapentin, tramadol and/or amitriptyline mixtures in biological fluids.
Ibrahim A NaguibNesma A AliFadwa A ElrobyMohamed R El GhobashyFatma F AbdallahPublished in: Bioanalysis (2020)
Background: Mixtures of gabapentin, tramadol and/or amitriptyline are usually recommended for treatment of neuropathic pain. Materials & methods/results: A novel GC-MS/MS method was developed to assess the studied mixture whether in pure forms or human biological fluids (plasma/urine). The chromatographic detection was performed using MS detector applying the selected ion-monitoring mode. An (Agilent, CA, USA) GC-MS with triple axis single quadrupole detector unit was used for the analysis equipped with HP-5MS (5% phenyl methyl siloxane) column. Helium was the carrier gas and positive electron impact ionization mode was applied. Conclusion: The developed method was able to assess the mixture components simultaneously within six minutes. Validation of the method was assured according to US FDA guidelines and Eco-Scale assessment.
Keyphrases
- neuropathic pain
- ms ms
- mass spectrometry
- spinal cord
- spinal cord injury
- multiple sclerosis
- endothelial cells
- liquid chromatography
- ionic liquid
- simultaneous determination
- high performance liquid chromatography
- image quality
- tandem mass spectrometry
- loop mediated isothermal amplification
- postoperative pain
- solar cells
- replacement therapy
- clinical evaluation