Regulation Mechanism of Dopamine Receptor 1 in Low Temperature Response of Marsupenaeus japonicus .
Xianyun RenXueqiong BianHuixin ShaoShaoting JiaZhenxing YuPing LiuJian LiJitao LiPublished in: International journal of molecular sciences (2023)
Dopamine receptors (DARs) are important transmembrane receptors responsible for receiving extracellular signals in the DAR-mediated signaling pathway, and are involved in a variety of physiological functions. Herein, the D1 DAR gene from Marsupenaeus japonicus ( MjDAD1 ) was identified and characterized. The protein encoded by MjDAD1 has the typical structure and functional domains of the G-protein coupled receptor family. MjDAD1 expression was significantly upregulated in the gills and hepatopancreas after low temperature stress. Moreover, double-stranded RNA-mediated silencing of MjDAD1 significantly changed the levels of protein kinases (PKA and PKC), second messengers (cyclic AMP (cAMP), cyclic cGMP, calmodulin, and diacyl glycerol), and G-protein effectors (adenylate cyclase and phospholipase C). Furthermore, MjDAD1 silencing increased the apoptosis rate of gill and hepatopancreas cells. Thus, following binding to their specific receptors, G-protein effectors are activated by MjDAD1 , leading to DAD1-cAMP/PKA pathway-mediated regulation of caspase-dependent mitochondrial apoptosis. We suggest that MjDAD1 is indispensable for the environmental adaptation of M. japonicus .
Keyphrases
- binding protein
- protein kinase
- cell cycle arrest
- induced apoptosis
- oxidative stress
- endoplasmic reticulum stress
- cell death
- signaling pathway
- pi k akt
- protein protein
- nitric oxide
- epithelial mesenchymal transition
- amino acid
- type iii
- copy number
- gene expression
- long non coding rna
- metabolic syndrome
- risk assessment
- liquid chromatography