Quantification of Drug Molecules in Live Single Cells Using the Single-Probe Mass Spectrometry Technique.
Ning PanShawna J StandkeNaga Rama KothapalliMei SunRyan C BensenAnthony W G BurgettZhibo YangPublished in: Analytical chemistry (2019)
Analyzing cellular constituents on the single-cell level through mass spectrometry (MS) allows for a wide range of compounds to be studied simultaneously. However, there is a need for quantitative single-cell mass spectrometry (qSCMS) methods to fully characterize drug efficacy from individual cells within cell populations. In this study, qSCMS experiments were carried out using the Single-probe MS technique. The method was successfully used to perform rapid absolute quantifications of the anticancer drug irinotecan in individual mammalian cancer cells under ambient conditions in real time. Traditional liquid chromatography/mass spectrometry (LC/MS) quantifications of irinotecan in cell lysate samples were used to compare the results from Single-probe qSCMS. This technique showcases heterogeneity of drug efficacy on the single-cell level.
Keyphrases
- mass spectrometry
- single cell
- liquid chromatography
- rna seq
- high resolution
- gas chromatography
- induced apoptosis
- high resolution mass spectrometry
- high throughput
- capillary electrophoresis
- high performance liquid chromatography
- tandem mass spectrometry
- cell cycle arrest
- living cells
- multiple sclerosis
- quantum dots
- stem cells
- air pollution
- oxidative stress
- drug induced
- pi k akt
- cell death
- sensitive detection
- emergency department
- solid phase extraction
- particulate matter