Manual mass spectrometry de novo sequencing of the anionic host defense peptides of the Cuban Treefrog Osteopilus septentrionalis.

Tatiana Y SamginaMaria D TolpinaAlexey K SurinSergey V KovalevRoberto Alonso Bosch Isel Pascual AlonsoFabiola Almeida GarciaLuis Javier Gonzalez LopezAlbert T Lebedev

Published in: Rapid communications in mass spectrometry : RCM (2021)

Mirror-symmetrical peptides are sequenced using N-terminal acetylation. Acetylated Ser is reliably distinguished from isomeric Glu by the loss of ketene from b-ions containing the corresponding residue. Calculations of the physicochemical and structural properties of the discovered anionic septenins 1 and 2 allowed the mechanism of their interaction with microbe cells to be postulated.

Keyphrases

mass spectrometry
induced apoptosis
amino acid
cell cycle arrest
liquid chromatography
density functional theory
molecular dynamics
single cell
high resolution
endoplasmic reticulum stress
quantum dots
cell death
high performance liquid chromatography
oxidative stress
innate immune
simultaneous determination