Novel Non-Viral Vectors Based on Pluronic ® F68PEI with Application in Oncology Field.
Inês SilvaCátia Sofia da Costa DominguesIvana JarakRui A CarvalhoRosemeyre A CordeiroMarília DouradoFrancisco VeigaHenrique FanecaAna FigueirasPublished in: Polymers (2022)
Copolymers composed of low-molecular-weight polyethylenimine (PEI) and amphiphilic Pluronics® are safe and efficient non-viral vectors for pDNA transfection. A variety of Pluronic® properties provides a base for tailoring transfection efficacy in combination with the unique biological activity of this polymer group. In this study, we describe the preparation of new copolymers based on hydrophilic Pluronic® F68 and PEI (F68PEI). F68PEI polyplexes obtained by doping with free F68 (1:2 and 1:5 w/w) allowed for fine-tuning of physicochemical properties and transfection activity, demonstrating improved in vitro transfection of the human bone osteosarcoma epithelial (U2OS) and oral squamous cell carcinoma (SCC-9) cells when compared to the parent formulation, F68PEI. Although all tested systems condensed pDNA at varying polymer/DNA charge ratios (N/P, 5/1−100/1), the addition of free F68 (1:5 w/w) resulted in the formation of smaller polyplexes (<200 nm). Analysis of polyplex properties by transmission electron microscopy and dynamic light scattering revealed varied polyplex morphology. Transfection potential was also found to be cell-dependent and significantly higher in SCC-9 cells compared to the control bPEI25k cells, as especially evident at higher N/P ratios (>25). The observed selectivity towards transfection of SSC-9 cells might represent a base for further optimization of a cell-specific transfection vehicle.
Keyphrases
- induced apoptosis
- cell cycle arrest
- endothelial cells
- sars cov
- palliative care
- signaling pathway
- stem cells
- risk assessment
- mesenchymal stem cells
- cell therapy
- climate change
- drug delivery
- electron microscopy
- bone marrow
- bone mineral density
- liquid chromatography
- pi k akt
- cell free
- soft tissue
- solid phase extraction
- tandem mass spectrometry