Highly Sensitive Love Mode Acoustic Wave Platform with SiO 2 Wave-Guiding Layer and Gold Nanoparticles for Detection of Carcinoembryonic Antigens.
Chong LiJikai ZhangHaiyu XieJingting LuoChen FuRan TaoHonglang LiYong Qing Richard FuPublished in: Biosensors (2022)
A highly sensitive and precise Love wave mode surface acoustic wave (SAW) immunosensor based on an ST-cut 90°X quartz substrate and an SiO 2 wave-guiding layer was developed to detect cancer-related biomarkers of carcinoembryonic antigens (CEAs). A delay line structure of the SAW device with a resonant frequency of 196 MHz was designed/fabricated, and its surface was functionalized through CEA antibody immobilization. The CEA antibodies were bound with gold nanoparticles and CEA antibodies to form a sandwich structure, which significantly amplified the mass loading effect and enhanced the maximum responses by 30 times. The center frequency of the Love wave immunosensor showed a linear response as a function of the CEA concentration in the range of 0.2-5 ng/mL. It showed a limit of detection of 0.2 ng/mL, and its coefficient of determination was 0.983. The sensor also showed minimal interference from nonspecific adsorptions, thus demonstrating its promise for point-of-care applications for cancer biomarkers.
Keyphrases
- gold nanoparticles
- label free
- molecularly imprinted
- dendritic cells
- sensitive detection
- squamous cell carcinoma
- computed tomography
- machine learning
- papillary thyroid
- high throughput
- high resolution
- magnetic nanoparticles
- big data
- quantum dots
- deep learning
- squamous cell
- solid phase extraction
- artificial intelligence
- neural network