The guide RNA sequence dictates the slicing kinetics and conformational dynamics of the Argonaute silencing complex.

Published in: bioRxiv : the preprint server for biology (2024)

Sequence of guide RNA can alter slicing rate of fully paired substrate by 250-foldSequences that cause more rapid slicing direct more efficient RNAi in cellsStrong central pairing imparts tolerance for mismatches to the guide 3' regionThis tolerance is attributable to more fully populating the slicing conformation.

Keyphrases

molecular dynamics simulations
amino acid
molecular dynamics
nucleic acid
crystal structure