Identification and characterization of an injury-induced skeletal progenitor.
Owen MarecicRuth TevlinAdrian McArdleEun Young SeoTaylor WeardaChristopher DuldulaoGraham G WalmsleyAllison NguyenIrving L WeissmanCharles K F ChanMichael T LongakerPublished in: Proceedings of the National Academy of Sciences of the United States of America (2015)
The postnatal skeleton undergoes growth, remodeling, and repair. We hypothesized that skeletal progenitor cells active during these disparate phases are genetically and phenotypically distinct. We identified a highly potent regenerative cell type that we term the fracture-induced bone, cartilage, stromal progenitor (f-BCSP) in the fracture callus of adult mice. The f-BCSP possesses significantly enhanced skeletogenic potential compared with BCSPs harvested from uninjured bone. It also recapitulates many gene expression patterns involved in perinatal skeletogenesis. Our results indicate that the skeletal progenitor population is functionally stratified, containing distinct subsets responsible for growth, regeneration, and repair. Furthermore, our findings suggest that injury-induced changes to the skeletal stem and progenitor microenvironments could activate these cells and enhance their regenerative potential.
Keyphrases
- stem cells
- gene expression
- high glucose
- diabetic rats
- mesenchymal stem cells
- preterm infants
- induced apoptosis
- drug induced
- type diabetes
- bone mineral density
- bone marrow
- endothelial cells
- cell fate
- risk assessment
- dna methylation
- signaling pathway
- climate change
- hip fracture
- bone loss
- peripheral blood
- childhood cancer
- gestational age
- wild type