Expansion of airway basal epithelial cells from primary human non-small cell lung cancer tumors.
Robert Edward HyndsAssma Ben AissaKate H C GowersThomas B K WatkinsLeticia Bosshard-CarterAndrew J RowanSelvaraju VeeriahGareth A WilsonSergio A QuezadaCharles Swantonnull nullSam M JanesPublished in: International journal of cancer (2018)
Pre-clinical non-small cell lung cancer (NSCLC) models are poorly representative of the considerable inter- and intra-tumor heterogeneity of the disease in patients. Primary cell-based in vitro models of NSCLC are therefore desirable for novel therapy development and personalized cancer medicine. Methods have been described to generate rapidly proliferating epithelial cell cultures from multiple human epithelia using 3T3-J2 feeder cell culture in the presence of Y-27632, a RHO-associated protein kinase (ROCK) inhibitor, in what are known as "conditional reprograming conditions" (CRC) or 3T3 + Y. In some cancer studies, variations of this methodology have allowed primary tumor cell expansion across a number of cancer types but other studies have demonstrated the preferential expansion of normal epithelial cells from tumors in such conditions. Here, we report our experience regarding the derivation of primary NSCLC cell cultures from 12 lung adenocarcinoma patients enrolled in the Tracking Cancer Evolution through Therapy (TRACERx) clinical study and discuss these in the context of improving the success rate for in vitro cultivation of cells from NSCLC tumors.
Keyphrases
- papillary thyroid
- small cell lung cancer
- single cell
- end stage renal disease
- squamous cell
- ejection fraction
- endothelial cells
- newly diagnosed
- cell therapy
- chronic kidney disease
- advanced non small cell lung cancer
- protein kinase
- squamous cell carcinoma
- prognostic factors
- lymph node metastasis
- patient reported outcomes
- bone marrow
- induced pluripotent stem cells
- brain metastases
- young adults
- case control