MoNap1, a Nucleosome Assemble Protein 1, Regulates Growth, Development, and Pathogenicity in Magnaporthe oryzae .
Shulin ZhangYu WangXinyue CuiJinmei HuXiaoru KangYuyan LiuYuemin PanPublished in: Journal of fungi (Basel, Switzerland) (2022)
Nap1 is an evolutionarily conserved protein from yeast to human and is involved in diverse physiological processes, such as nucleosome assembly, histone shuttling between the nucleus and cytoplasm, transcriptional regulation, and the cell cycle regulation. In this paper, we identified nucleosome assemble protein MoNap1 in Magnaporthe oryzae and investigated its function in pathogenicity. Deletion of MoNAP1 resulted in reduced growth and conidiation, decreased appressorium formation rate, and impaired virulence. MoNap1 affects appressorium turgor and utilization of glycogen and lipid droplets. In addition, MoNap1 is involved in the regulation of cell wall, oxidation, and hyperosmotic stress. The subcellular localization experiments showed that MoNap1 is located in the cytoplasm. MoNap1 interacts with MoNbp2, MoClb3, and MoClb1 in M. oryzae . Moreover, deletion of MoNBP2 and MoCLB3 has no effects on vegetative growth, conidiation, and pathogenicity. Transcriptome analysis reveals that MoNAP1 is involved in regulating pathogenicity, the melanin biosynthetic process. Taken together, our results showed that MoNap1 plays a crucial role in growth, conidiation, and pathogenicity of M. oryzae .
Keyphrases
- cell cycle
- biofilm formation
- cell wall
- cell proliferation
- escherichia coli
- binding protein
- staphylococcus aureus
- pseudomonas aeruginosa
- gene expression
- dna methylation
- transcription factor
- amino acid
- protein protein
- mass spectrometry
- genome wide
- nitric oxide
- antimicrobial resistance
- hydrogen peroxide
- high resolution
- single molecule
- rna seq