Molecular Engineering Powered Dual-Readout Point-of-Care Testing for Sensitive Detection of Escherichia coli O157:H7.
Gan ZhangZhen HuangLiwen HuYumeng WangShengliang DengDaofeng LiuJuan PengWei-Hua LaiPublished in: ACS nano (2023)
Escherichia coli O157:H7 ( E. coli O157:H7) has become one of the major threats to public health and food safety. However, the culture method as a gold standard for the detection of E. coli O157:H7 requires laborious operations and a long processing time. Herein, we developed a dual-readout aggregation-induced emission nanoparticle-based lateral flow immunoassay (LFIA) for sensitive detection of E. coli O157:H7 to achieve a qualitative and quantitative assay for satisfying the applications under varying scenarios. 2,3-Bis(4-(bis(4-( tert -butyl)phenyl)amino)phenyl)fumaronitrile (BAPF), an aggregation-induced emission luminogen, was designed to achieve a strong molar extinction coefficient (3.0 × 10 4 M -1 cm -1 ) and high quantum yield (33.28%), which was further verified by a large rotation angle and low energy gap. Subsequently, BAPFs were integrated into a nanostructured system to form excellent water-soluble nanoparticles (BAPFNPs) for the detection of E. coli O157:H7 with colorimetric and fluorescent readout. The designed BAPFNPs-based LFIA (BAPFNPs-LFIA) exhibited nearly qualitative ability with gold nanoparticles-LFIA (AuNPs-LFIA) and a 9 times enhancement compared with quantum beads-LFIA (QBs-LFIA) in quantitative aspect. Especially, FL-BAPFNPs-LFIA could detect E. coli O157:H7 earlier than QBs-LFIA and AuNPs-LFIA when samples with low E. coli O157:H7 concentrations were cultured. Overall, the proposed strategy revealed that versatile BAPFNPs have great potential as reporters for dual-readout ability and enhancing detection sensitivity for rapid and accurate pathogenic bacteria assay.
Keyphrases
- sensitive detection
- escherichia coli
- loop mediated isothermal amplification
- quantum dots
- gold nanoparticles
- public health
- label free
- high resolution
- klebsiella pneumoniae
- water soluble
- biofilm formation
- high throughput
- molecular dynamics
- real time pcr
- computed tomography
- human health
- climate change
- staphylococcus aureus
- magnetic resonance imaging
- single cell
- endothelial cells
- hydrogen peroxide
- pseudomonas aeruginosa
- single molecule
- energy transfer
- reduced graphene oxide