Multiresidue Determination of the Anabolic-Agent Residues Steroids, Stilbenes, and Resorcylic Acid Lactones in Bovine Urine by GC-MS/MS with Microwave-Assisted Derivatization.
Amanda L SilveiraMauro Lúcio G de OliveiraDiego G RochaSérgio DraczThiago F BorgatiMary Ane G LanaRodinei AugustiAdriana Ferreira FariaPublished in: Journal of agricultural and food chemistry (2018)
In this work, a GC-MS/MS method was developed for the determination of anabolic-agent residues in bovine urine. The optimized sample preparation was as follows: enzymatic hydrolysis by β-glucuronidase-sulfatase enzyme from Helix pomatia for 16 h at 37.5 °C, liquid-liquid extraction with diethyl ether, solid-phase extraction with HLB and aminopropylsilane cartridges, and microwave-assisted derivatization using 25 μL of MSTFA/NH4I/ethanethiol and full microwave power for 2 min. The method was validated according to Decision 657/2002/EC, Codex Alimentarius, and Manual da Garantia da Qualidade Analítica guidelines. The acceptability criteria for quantitative analysis were met for α-ethinylestradiol, α-nandrolone, β-estradiol, β-zearalanol, β-zearalenol, drostanolone, ethisterone, dienestrol, diethylstilbestrol, hexestrol, megestrol, methyltestosterone, and zearalenone. The analytes α-zearalenol, α-zearalanol, and norethandrolone were validated for qualitative analysis.
Keyphrases
- solid phase extraction
- liquid chromatography tandem mass spectrometry
- gas chromatography
- molecularly imprinted
- tandem mass spectrometry
- high performance liquid chromatography
- ms ms
- ultra high performance liquid chromatography
- simultaneous determination
- gas chromatography mass spectrometry
- liquid chromatography
- high resolution mass spectrometry
- mass spectrometry
- high resolution
- hydrogen peroxide
- tyrosine kinase
- estrogen receptor
- systematic review
- room temperature
- clinical practice