Siphon-Controlled Automation on a Lab-on-a-Disc Using Event-Triggered Dissolvable Film Valves.
Brian D HendersonDavid J KinahanJeanne RioRohit MishraDamien KingSarai M Torres-DelgadoDario MagerJan G KorvinkJens DucréePublished in: Biosensors (2021)
Within microfluidic technologies, the centrifugal microfluidic "Lab-on-a-Disc" (LoaD) platform offers great potential for use at the PoC and in low-resource settings due to its robustness and the ability to port and miniaturize 'wet bench' laboratory protocols. We present the combination of 'event-triggered dissolvable film valves' with a centrifugo-pneumatic siphon structure to enable control and timing, through changes in disc spin-speed, of the release and incubations of eight samples/reagents/wash buffers. Based on these microfluidic techniques, we integrated and automated a chemiluminescent immunoassay for detection of the CVD risk factor marker C-reactive protein displaying a limit of detection (LOD) of 44.87 ng mL-1 and limit of quantitation (LoQ) of 135.87 ng mL-1.
Keyphrases
- label free
- high throughput
- room temperature
- circulating tumor cells
- single cell
- aortic valve
- risk factors
- loop mediated isothermal amplification
- aortic valve replacement
- ms ms
- machine learning
- mass spectrometry
- heart failure
- deep learning
- density functional theory
- liquid chromatography tandem mass spectrometry
- gold nanoparticles
- single molecule
- robot assisted
- minimally invasive
- transcatheter aortic valve implantation