Long-term culture of mesenchymal stem cells impairs ATM-dependent recognition of DNA breaks and increases genetic instability.

Daniela HladikInes HöfigUrsula OestreicherJohannes BeckersMartina MatjanovskiXuanwen BaoHarry ScherthanMichael J AtkinsonMichael Rosemann

Published in: Stem cell research & therapy (2019)

The detrimental impact of long-term in vitro expansion on DNA damage response of MSCs warrants a regular monitoring of this process during the ex vivo growth of these cells to improve therapeutic safety and efficiency.

Keyphrases

dna damage response
mesenchymal stem cells
dna repair
umbilical cord
induced apoptosis
dna damage
bone marrow
cell cycle arrest
circulating tumor
genome wide
single molecule
copy number
stem cells
cell free
cell therapy
signaling pathway
oxidative stress
nucleic acid