Plasmonic SERS Au Nanosunflowers for Sensitive and Label-Free Diagnosis of DNA Base Damage in Stimulus-Induced Cell Apoptosis.
Guohua QiDandan WangChuanping LiKongshuo MaYing ZhangYongdong JinPublished in: Analytical chemistry (2020)
Molecular diagnosis and accurate damage analysis of complex genomic DNAs in tumor cells are crucial to the theranostics of cancers but still a huge challenge. Herein, by designed preparation of a uniform plasmonic sunflower-like assembly gold (Au) nanostructure that is capable of efficient DNA capture and providing high-density gap-plasmon "hot spots" for adequate surface-enhanced Raman spectroscopy (SERS) enhancement, we succeeded in sensitive and reliable label-free SERS detection of DNA damage in electrostimulus-induced apoptotic cancer cells at the DNA base level for the first time. The SERS results showed that the external electrostimulus (at 1.2 V, for 5 min) was almost harmless to normal healthy cells, but it caused pronounced double strand break and adenine base damage in cancer cell DNAs, which effectively destroyed the reproduction and transcription of DNAs and ultimately induced cell apoptosis. The developed sensing platform and method are promising for cell study of genetically related diseases.
Keyphrases
- quantum dots
- label free
- sensitive detection
- raman spectroscopy
- single molecule
- oxidative stress
- diabetic rats
- high glucose
- dna damage
- high density
- circulating tumor
- cell proliferation
- cell free
- gold nanoparticles
- drug induced
- stem cells
- cell death
- single cell
- cell therapy
- nucleic acid
- dna repair
- transcription factor
- high throughput
- cell cycle arrest
- copy number