Determination of tigecycline in human lung epithelial cells and polymorphonuclear neutrophils by liquid chromatography/tandem mass spectrometry and its application in a cellular pharmacokinetics study.
Qianting YangJiao XieHaitao WangYanping ZhangYang ZhaoSha LiKanghuai ZhangYa-Lin DongPublished in: Rapid communications in mass spectrometry : RCM (2021)
Validated LC/MS/MS is a simple, rapid, and sensitive method for determining the intracellular concentration of tigecycline, and tigecycline has good penetrations both in human BEAS-2B cells and PMNs. The method can be efficiently used for future studies of the intracellular pharmacokinetics of tigecycline.
Keyphrases
- acinetobacter baumannii
- liquid chromatography tandem mass spectrometry
- klebsiella pneumoniae
- solid phase extraction
- multidrug resistant
- drug resistant
- simultaneous determination
- endothelial cells
- pseudomonas aeruginosa
- ms ms
- reactive oxygen species
- molecularly imprinted
- current status
- induced pluripotent stem cells
- tandem mass spectrometry
- loop mediated isothermal amplification
- sensitive detection
- quantum dots