Microbial ecology in selenate-reducing biofilm communities: Rare biosphere and their interactions with abundant phylotypes.

Selenate (SeO4 2- ) reduction in hydrogen (H2 )-fed membrane biofilm reactors (H2 -MBfRs) was studied in combinations with other common electron acceptors. We employed H2 -MBfRs with two distinctly different conditions: R1, with ample electron-donor availability and acceptors SeO4 2- and sulfate (SO4 2- ), and R2, with electron-donor limitation and the presence of electron acceptors SeO4 2- , nitrate (NO3 - ), and SO4 2- . Even though H2 was available to reduce all input SeO4 2- and SO4 2- in R1, SeO4 2- reduction was preferred over SO4 2- reduction. In R2, co-reduction of NO3 - and SeO4 2- occurred, and SO4 2- reduction was mostly suppressed. Biofilms in all MBfRs had high microbial diversity that was influenced by the "rare biosphere" (RB), phylotypes with relative abundance less than 1%. While all MBfR biofilms had abundant members, such as Dechloromonas and Methyloversatilis, the bacterial communities were significantly different between R1 and R2. For R1, abundant genera were Methyloversatilis, Melioribacter, and Propionivibrio; for R2, abundant genera were Dechloromonas, Hydrogenophaga, Cystobacter, Methyloversatilis, and Thauera. Although changes in electron-acceptor or -donor loading altered the phylogenetic structure of the microbial communities, the biofilm communities were resilient in terms of SeO4 2- and NO3 - reductions, because interacting members of the RB had the capacity of respiring these electron acceptors.