Metabolic Study of Cancer Cells Using a pH Sensitive Hydrogel Nanofiber Light Addressable Potentiometric Sensor.
Parmiss Mojir ShaibaniHashem EtayashSelvaraj NaickerKamaljit KaurThomas ThundatPublished in: ACS sensors (2017)
We report a simple, fast, and cost-effective approach that measures cancer cell metabolism and their response to anticancer drugs in real time. Using a Light Addressable Potentiometric Sensor integrated with pH sensitive hydrogel nanofibers (NF-LAPS), we detect localized changes in pH of the media as cancer cells consume glucose and release lactate. NF-LAPS shows a sensitivity response of 74 mV/pH for cancer cells. Cancer cells (MDA MB231) showed a response of ∼0.4 unit change in pH compared to virtually no change observed for normal cells (MCF10A). We also observed a drop in pH for the multidrug-resistant cancer cells (MDA-MB-435MDR) in the presence of doxorubicin. However, inhibition of the metabolic enzymes such as hexokinase and lactate dehydrogenase-A suggested an improvement in the efficacy of doxorubicin by decreasing the level of acidification. This approach, based on extracellular acidification, enhances our understanding of cancer cell metabolic modes and their response to chemotherapies, which will help in the development of better treatments, including choice of drugs and dosages.
Keyphrases
- multidrug resistant
- drug delivery
- breast cancer cells
- cell cycle arrest
- signaling pathway
- pi k akt
- induced apoptosis
- oxidative stress
- drug resistant
- cancer therapy
- nuclear factor
- cell death
- gram negative
- hyaluronic acid
- escherichia coli
- blood pressure
- blood glucose
- adipose tissue
- klebsiella pneumoniae
- toll like receptor